Vol. 12, Special Issue 8 (2023)

Author(s):
Durga Veera Hanuman Donga, Venkata Rao K V, Bhaskar Debbarma, Anil Kumar Banothu, Rajendar Bobbili, Sravathi Vemula, Venkatesh Vankudothu, Atchuta Rao Mullu, Vamshi Kiran Amgoth and Anjaneyulu Potluri

Abstract:
Oxidative stress is one of the foremost cellular damages occurring in many of the disease conditions. Among the different organelles, the erythrocytes are more prone to free radical damage. Annona squamosa contain high antioxidant activity as it is a rich source of carotenoids, polyphenolic acids and flavonoids. In the current study, the antioxidant action of Annona squamosa in sodium fluoride-induced oxidative stress in sheep RBC was studied. RBCs are more susceptible to oxidative stress because their membrane contains more polyunsaturated fatty acids. Sodium fluoride (NaF) was used to induce oxidative stress in red blood cells by incubating them for 24 hours, ascorbic acid and annona squamosa were used to protect them from oxidative stress. The altered amounts of SOD, GSH and MDA in RBC were used to assess oxidative stress. Treatment with ascorbic acid and Annona squamosa extract reverted the proportions of SOD, GSH and MDA to normal. Finally, ascorbic acid and Annona squamosa can alleviate the oxidative stress caused by sodium fluoride (NaF) in erythrocytes.