Vol. 12, Special Issue 8 (2023)

Author(s):
Amrutha VS, Vinodkumar K, Vijayakumar K, Sulficar S, Priya PM and Jithil VR

Abstract:
Present study was done for the detection of Cryptosporidium induced diarrhoea in neonatal calves using Nested PCR technique. Neonatal calf diarrhoea is an economically important disease in dairy sector which is present all over the world. Both infectious and non-infectious etiologies are reported. Major infectious etiological agents include rota virus, corona virus, E. coli and Cryptosporidium parvum. Using staining techniques it was able to detect the presence of Cryptosporidium in five out of 50 animals with diarrhoea but the Nested PCR technique was helped in diagnosing 19 positive cases among the same group of 50 animals. Hence this is a more sensitive technique for the advanced diagnosis of Cryptosporidiosis.