Vol. 12, Special Issue 8 (2023)

Author(s):
Kaviraj Airani, Renukaradhya GJ, Sahadev A, Naveenkumar S, Sathisha KB and Ningaraju K

Abstract:
The present study was undertaken with the objective of establishing the normal spermiogram in Gir bulls and sperm motion kinematics using CASA in their non-breeding tract. About 60 semen ejaculates from three Gir bulls in four months period were collected by AV method and colour of the ejaculates was creamy to milky white and consistency with mean value of 2.80±0.08 (out of 4). The observed overall mean ejaculate volume (mL), mass motility (0-5 scale), individual motility (%), sperm concentration (106/mL), sperm viability (%), sperm abnormality (%), HOST reacted (%), acrosome integrity and post thaw motility were 4.91±0.22, 3.40±0.06, 82.40±0.45, 900±34.90, 88.27±0.20, 11.87±0.19, 87.27±0.34, 90.80±0.28 and 57.03±0.51, respectively. The sperm kinematics parameters viz., VCL, VSL and VAP were 119.90±1.68,76.11±1.55 and82.30±1.37 in fresh semen and 100.70±1.58, 60.64±1.50 and 67.53±1.34 µm/sec inpost thaw values, respectively and are similar to those of native tract.