Vol. 12, Special Issue 8 (2023)

Author(s):
Deepesh Sharma, Stuti Vatsya, Rajeev Ranjan Kumar and Sudhir Kumar

Abstract:
Bovine tropical theileriosis is a deadly tick borne disease of large ruminants caused by Theileria annulata. The aim of this study was to detect T. annulata in large ruminants of Uttarakhand state and comparison of microscopic and PCR technique. A total of 1006 blood samples of suspected animals for theileriosis across the Uttarakhand state. All the samples were initially screened for theileriosis by microscopic examination using Giemsa’s staining technique and by doing Polymerase Chain Reaction (PCR) targeting 1098 bp fragment of Tams1 gene. Total of 209(20.77%) and 283 (28.13%) of blood samples were found positive for theileriosis by microscopic and PCR technique respectively. The study showed that sensitivity and specificity of TBE was 73.85% and 100%, respectively while the same of PCR assay were 100%.