Vol. 12, Special Issue 8 (2023)

Author(s):
Jayanthi KV, Jayanth KV, Akshata Patil, Chandrika MR, Sahana VN, Ganesh, Chandana Sree Chinnareddyvari, Preeti and Rajeshwari

Abstract:
DNA the genetic material is unique to every organism. Identification of individual, detection of cause for diseases, in the field of selection of animals for breeding etc., evaluation of individual is very important and genotypic evaluation gives us accurate results. Therefore, in genetics molecular techniques and its utility has raised tremendously. This paper is written with an objective to through light on readers about few basic molecular techniques like DNA isolation, PCR, Agarose gel electrophoresis, Restriction enzymes, Cloning, recombinant DNA technology, hybridization, blotting and transgenic operations in a single go. The mechanism of action, principles of techniques, pros and cons of basic molecular technique along with its utility is detailed in this article.