Vol. 12, Special Issue 8 (2023)

Material and methods: 100 samples were collected from the ILFC (Instructional Livestock Farm Complex Veterinary College) in and around Bidar district (Navbad and Kamthana). According to Paul et al. (2010), E. coli isolates were isolated and identified. E. coli isolates that had been identified culturally were also tested for metabolic profile (biochemical) and antibiotic sensitivity test.

Result: 85 (85%) of the 100 samples that were collected were positive for E. coli species. Each of the 100 samples was inoculated into nutrient broth and left to incubate for 24 hours at 37 °C. A loop of nutrient broth was selectively plated into MacConkey agar after 24 hours, and looked for the appearance of small, pinpoint pink colonies. The pinpoint pink coloured colonies were then streaked onto eosin methylene blue agar, where the appearance of colonies with black centres and metallic sheen was watched for. The prevalence E. coli was reported to be present 85% (85/100). E. coli isolates showed sensitivity for antibiotics like Ciprofloxacin, Co-trimoxazole, and Ceftriaxone-tazobactum. Further, E. coli isolates were resistant to penicillin, streptomycin, erythromycin, kanamycin, amoxyclav, and amoxicillin were the most common.