Vol. 12, Special Issue 9 (2023)

The present study was conducted to evaluate the effect of aflatoxin and ochratoxin on serum biochemical changes and their amelioration using adsorbents in birds. Around 128 one day age broiler chicks were taken and divided into 8 birds in each group total amounting to 4 groups with 4 replicates in a complete randomized design. Each group was fed for 6 weeks with four different diets viz Group 1: Toxin free basal diet (BD), (Control), group 2: A mixture of BD, aflatoxin (AF) and ochratoxin A (OA). Group 3: Mixture of BD, AF, OA and activated charcoal (AC) and Group 4: Mixture of basal diet, AF, OA, AC and lyophilized yeast culture (Yc). The whole blood was collected on 14^th, 28^th and 42^nd day and serum were separated to evaluate the biochemical parameters. The result indicated a significant decrease in the glucose, total proteins, albumin, globulin, cholesterol, triglycerides, calcium, phosphorus and significant increase in the bilirubin in group 2 when compared to control group. Group 3 birds showed significant improvement in the biochemical profile when compared with group 2 and varying significantly from groups 4 and 1, which indicates that AC (@ 0.4%) has only partial amelioration effect on combined toxicity. Group 4 showed significant improvement when compared to group 2 and 3. The results obtained from the present study indicated that only AC has partial amelioration where as a combination of AC (@ 0.4%) and yeast culture (@ 0.2%) has complimentary effect in amelioration of the combined toxicity in broilers. However, the combination of the adsorbents cannot completely ameliorate the combined toxicity when compared to control group.