Vol. 12, Special Issue 9 (2023)

Author(s):
A Suvarna, D Anil Pavan Kumar, P Jaya Laxmi and RMV Prasad

Abstract:
Using ten microsatellite markers, the genetic diversity of the local sheep that are common in Telangana's Nagarjuna Sagar region were genotyped. Ten microsatellite markers and a total of 82 alleles were examined. OarAE129 and OarCP38 had the fewest alleles (6 each), whereas MAF214 had the most alleles (11), with a mean of 8.2 alleles. With a mean value of 7.221, the effective number of alleles ranged from 4.810 (OarAE129) to 9.970 (MAF214). While the allele frequencies ranged from a minimum of 0.050 (BM8125, INRA063, MAF214) to a maximum of 0.315 (OarAE129), the allele sizes varied from a minimum of 112bp in OarCP38 to a maximum of 264bp in MAF214.The mean heterozygosity that was observed and predicted, respectively, was 0.104 and 0.855. The mean inbreeding coefficient across the board was 0.878. The F_IS values were between 0.832 to 0.941 (OarCP34 to BM8125). The study's inbreeding estimations were all positive, indicating a severe shortage of heterozygotes. The PIC values for the ten microsatellite loci varied from 0.762 (OarAE129) to 0.890 (MAF214) and all ten loci (100%) were determined to be highly polymorphic. It was discovered that the mean PIC value across all ten loci was 0.836. The chi-square test showed that all 10 loci were significantly deviating from the Hardy-Weinberg equilibrium adding to our understanding of the genetic makeup of this indigenous sheep. High genetic variability within the breed was found by the results.