Vol. 12, Special Issue 9 (2023)

Author(s):
Niranjan D, Shridhar NB, Vinuta MH, Sunilchandra U, Manjunatha SS, Pradeep BS, Manju GU and Patel Suresh Revanna

Abstract:
By using the FRAP (Ferric reducing antioxidant power assay) and DPPH (2, 2-diphenyl-1-picrylhydrazyl) assays, a study was conducted to evaluate the potential in vitro antioxidant activity of the methanolic extract of T. orientalis. The experiments were carried out using aliquots of methanolic extract of T. orientalis (METO) in various doses. In an UV spectrophotometer, absorbance was measured at 517 nm using ascorbic acid as the reference. The parameters for the antioxidant activity are found using the spectrophotometric measurement of the color change. When compared to ascorbic acid, which served as the reference, the in vitro antioxidant analysis using the ferric reducing antioxidant test and the DPPH assay at 200 µg/ml and 250 µg/ml doses of METO demonstrated minimal antioxidant activity. Since most tannins and flavonoids are phenolic compounds, they may be responsible for METO's antioxidant properties. The solvent and plant materials used in the antioxidant analysis study contributed a portion to METO's lower antioxidant potency compared to standards. T. orientalis has been found to have reduced antioxidant potential, which could be further investigated to assess the therapeutic antioxidant efficacy in domestic animals in clinical circumstances.