Vol. 12, Special Issue 9 (2023)

Author(s):
Chandravathi T, Rama Devi V, Satheesh K, Ravi Kumar P and Sudhakar K

Abstract:
Fowl cholera was caused by gram negative bacteria Pasteurella multocida. Thirty birds from private poultry farm suffering with pyrexia were brought to the Department of Veterinary Pathology, NTR College of Veterinary science, Gannavaram, Andhra Pradesh. Birds were dehydrated and emaciated and showing pyrexia, pale combs, mucus discharge from nostrils and greenish white coloured diarrhoea. On necropsy examination haemorrhages were observed on abdominal fat, epicardium, caecal tonsils, and intestinal mucosa. Lungs were congested. Liver was swollen, congested and containing multiple small focal areas of necrosis. The impression smears from liver and blood smears from heart in on Leishman’s staining revealed characteristic bipolar organisms. Histopathological studies of heart revealed congestion, haemorrhages, myocardial degeneration and liver showed congestion of central vein and sinusoids and mild degree of degenerative changes and focal areas of necrosis with heterophils and mononuclear cell infiltration. The disease was confirmed by amplifying species specific KMT gene through PCR which yielded 460bp size amplicon and cap A capsule-specific primers for capsular genotyping yielded 1044bp size amplicon. Based on bacterial isolation, blood smear examination and pathological lesions, disease was diagnosed and confirmed by molecular study.