Vol. 12, Special Issue 9 (2023)

Author(s):
Chandravathi T, Rama Devi V, Satheesh K, Ravi Kumar P and Sudhakar K

Abstract:
This study was conducted to investigate the concurrent presence of Escherichia coli (E. coli) bacteria in chicken suffering from the respiratory disease. Avian pathogenic E. coli (APEC) were detected based on isolation of bacteria, it’s cultural and colony characteristics and confirmed by polymerase chain reaction. Sixty isolates were confirmed as E. coli based on bacteriological isolation and molecular detection. Further, PCR assay was conducted for four virulent genes i.e. iss, vat, papC, and tsh and 54(90%) were positive on PCR for one or more virulent genes. Out of 60 isolates, 48 (80%) were found positive for iss gene, 43 (71.6%) positive for vat gene, 31 (51.6%) positive for tsh gene and 18 (30%) positive for papC gene. Two or more virulent genes were found in 46 (76.6%) isolates.