Vol. 12, Special Issue 9 (2023)

Author(s):
Bhargav K Kansagra, Deendayal Garg, Sneh D Patel, Tapas K Patbandha, Harish H Savsani, Jignesh A Chavda and Kirti R Makwana

Abstract:
The present study was carried out with the objective of to study the effect of incorporation of exogenous fibrolytic enzymes on in vitro degradability of cotton straw based total mixed ration. IVDMD significantly (p<0.05) increased @ 100000 & 200000 IU/kg cellulase and @ 50000 & 100000 IU/kg xylanase when supplemented alone. All levels of xylanase showed significant (p<0.05) effect on IVOMD as compared to control when supplemented alone. Cellulase also showed significant (p<0.05) effect @ 100000 & 200000 IU/kg and in combination @ 100000 IU/kg showed numerically higher IVOMD. IVTGP significantly (p<0.05) increased @ 100000 IU/kg xylanase and all combination of xylanase with 50000 IU/kg cellulase compared to control. Numerically higher gas production was observed in combination of enzymes @ 100000 IU/kg. TDOMR significantly (p<0.05) increased in combination @ 100000 and 50000 IU/kg cellulase and xylanase, respectively and also @ 200000 IU/kg of both enzymes. SCFA was significantly (p<0.05) improved @ 100000 IU/kg xylanase and combination of both enzymes @ 100000 IU/kg as well as in combination @ 100000 & 200000 IU/kg cellulase and xylanase, respectively. TVFA was numerically higher in combination @ 200000 IU/kg of both enzymes. NH3-N was numerically higher @ 200000 IU/kg xylanase.