Vol. 12, Special Issue 9 (2023)

Author(s):
Divyaprakash R Choravada, Praveen Singh, Owais Khan, Basavaraj Sajjanar, Ravi Kumar Gandham, Juli Chakma, Singh VK, Hemanth Kumar Pundla, Yogendra Pal and Mohd Tanzeel Aalam

Abstract:
Nucleosides, the fundamental building blocks of RNA and DNA, play pivotal roles in various cellular processes. Understanding their composition and potential modifications is crucial for unraveling intricate biological mechanisms. This study presents a comprehensive HPLC analysis of five key nucleosides: adenosine, guanosine, uridine, cytosine, and N6-methyladenosine (m6A). The chromatographic peaks revealed different retention time that offered insights into the nature of these nucleosides and their potential modifications by injecting a concentration of 30 μM. The method proved effective in separating and identifying these nucleosides at a level of 0.0566 µg- 0.5662 µg, underscoring its utility in quantification and characterization. Notably, co-elution peaks hinted at potential impurities or modifications, highlighting the complexity of these nucleoside samples. Moreover, this research illustrates the versatility of HPLC in analyzing nucleosides and modified forms, contributing to our understanding of their roles in various biological processes.