Vol. 12, Special Issue 9 (2023)

Author(s):
Navya Sri Bairi, CV Savalia, R Kumar and IH Kalyani

Abstract:
The present research work was carried out in the city of Navsari, Gujarat State, with the aim to isolate and identify Escherichia coli from meat of various species of animals using standard isolation methods followed by confirmation with PCR as well judging antibiotic sensitivity pattern of the isolates. During the study a total of 270 samples were processed, which included 210 meat samples (comprised of 60 samples of chicken, and 50 samples each, of chevon, mutton and carabeef) as well as 60 swab samples (20 samples each, of butcher’s knives, butchers’ hands and meat chopping board). Among 270 samples 63(23.3%) yielded growth of E. coli; which included 51(24.28%) from meat and 12(20%) from swab samples. The meat variety-wise spread of isolates showed that 14/60 (23%), 11/50 (22%), 8/50 (16%) and 18/50 (36%) samples were positive, respectively in case of chicken, chevon, mutton and carabeef. Antibiotic sensitivity test of all 63 isolates was performed using 11 antibiotics by agar disc diffusion method. The isolates exhibited 100% resistance towards Penicillin followed in descending order by Erythromycin (93.7%), Streptomycin (85.71%), Amikacin (77.7%) and Gentamicin (63.49%). An intermediate sensitivity/resistance was noticed against Amoxicillin (69.8%). While, the highest sensitivity of 92.5% was reported to Tetracycline followed by 84.12% against each, Norfloxacin and Ciprofloxacin, 82.5% to Chloramphenicol and, Enrofloxacin showed the least sensitivity of 68.25%.