Vol. 12, Special Issue 10 (2023)

Author(s):
Priya Singh, PR Tank, Shubham Janbandhu, Siddharth Kumar Jatav, Qurratul An Qureshi and Nidhi Dhansukhbhai Patel

Abstract:
This investigation was designed to evaluate the impact of white button mushrooms on disease resistance of white leg shrimp against Vibrio parahaemolyticus. From March to May 2022, the experiment was carried out at the College of Fisheries Science, Kamdhenu University, Veraval. Total six experimental diets with a 35% protein level were prepared. The control diet was prepared without incorporation of white button mushroom (WBM), while the treatment diets were prepared with incorporation of WBM powder at rates of 1%, 1.5%, 2%, 2.5% and 3% level. The post larvae of L. vannamei (average weight 0.012g) were stocked in each of 24 aquarium tanks (35 litres) at a density of 20 numbers, and they were fed with a treatment diet for 60 days. The four-day challenge test was conducted. For this purpose, shrimps were randomly selected (10 nos.) from each treatment and gently injected intramuscularly with 0.1x10⁴ cfu/ml of V. parahaemolyticus. The data pertaining to disease resistance of L. vannamei against V. parahaemolyticus were compiled and assessed. According to the study's findings, adding 3% WBM powder to the diet considerably increased the rate of survival following the challenge test against V. parahaemolyticus. The less mortality rate (10^d±4.08) was observed in the L. vannamei fed with the treatment diet (T₅) that was supplemented with 3% WBM powder while highest mortality rate (60^a±4.08) was found in control group (T₀). This suggests that white button mushrooms may have an effect on boosting the immune system of white leg shrimp that reduced the number of deaths brought on by V. parahaemolyticus infection. The result suggests that white button mushroom can be used as an effective feed ingredient that can help shrimp to fight against bacterial diseases in farming practices.