Vol. 12, Special Issue 10 (2023)

Author(s):
Ramya K, Gunaseelan L, Geetha M and K Sukumar

Abstract:
CanAntibiotic Quick Select Kit is an in vitro diagnostic assay that helps to select the correct antibiotic for clinical application in canines. The present study was undertaken in healthy as wells as diseased 25 clinical samples of dog and other species brought to Veterinary Clinical Complex, Namakkal during the year 2015 to select the appropriate antibiotic to treat the clinical conditions using the rapid field test kit. Twenty-five clinical samples collected respectively from dog (15), cattle (5), sheep (3) and poultry (2) were tested using the kit. The samples were tested against eight antibiotics viz., ciprofloxacin, ceftriaxone, ceftriaxone and tazobactum combination, ceftiofur, enrofloxacin, ampicillin and potassium clavulanate combination, ampicillin and dicloxacillin combination and finally amoxicillin, cloxacillin combination. Surprisingly, out of the twenty-five samples tested, 80 percent (12/15) from dog and 60 percent (3/5) from cattle were found to be resistant to penicillin group of antibiotics, 66.6 percent (2/3) from sheep and 50 percent (1/2) poultry were resistant to both penicillin group and fluoroquinolone group (ciprofloxacin and enrofloxacin). Simultaneously, the samples subjected to isolation indicated the presence of Staphylococcus pseudintermedius, Staphylococcus aureus, Streptococcus spp, E.coli Pseudomonas spp. and Klebsiella spp. in more than 90 percent of the samples. The results of the nanoparticle based kit indicate that it can be effortlessly relied while choosing the antibiotics for the therapeutic management in field conditions.