Vol. 12, Special Issue 11 (2023)

Author(s):
Raju Ram, Gowhar Iqbal, Jaspreet Singh, Devarshi Ranjan, Vikas Pathak, Shyam Kumar and Amrita Mohanty

Abstract:
Global gene expression profiling using RNA-seq, enabled by high throughput third-generation sequencing technology, revolutionizes transcriptome exploration. Compared to microarray, RNA-seq has advantages, including a larger dynamic range, accurate transcript quantification, and comprehensive tissue expression coverage. In addition to quantification of the transcript, RNA-seq is used for transcriptome comparison, mapping the both 5' and 3' ends of introns and mRNA, discovery of new exons, research into allele-specific expression, SNP finding, construction of de novo gene models and investigation into non-coding RNA. RNA-seq technology is increasingly utilized in various commercial and ecologically important species-related research for differential gene expression study and SNP discovery. The present review article encapsulates the RNA-seq profiling steps and potential applications in aquaculture.