Vol. 12, Special Issue 11 (2023)

Author(s):
Pushpalatha K, Shreya Talan and Dr. Raghu HV

Abstract:
Bovine mastitis is inflammation of the mammary gland caused by multiple pathogens with huge economic loss to the dairy industry. Indiscriminate use of antibiotics to treat such diseases in animals has led to the emergence of drug-resistant bacteria like E.coli, Staphylococcus aureus etc. Transmission of these AMR pathogens in the food chain continues to be a matter of great concern globally. In current study, The NDRI-developed Enzyme strip-based assay was used for evaluating 120 milk samples for their mastitis profile. Out of 120 samples, 54 samples were found to be normal as indicated by no change in colour, 45 samples were found to be subclinical and 21 were Clinical mastitis milk samples as indicated by intensity of colour development on strip. Further, the assay results were validated through conventional methods including CMT score and Somatic cell counter which confirmed the accuracy of strip based technology with neither false positive nor false negative results.