Vol. 12, Special Issue 11 (2023)

Author(s):
Kiran, Bharti Yadav, Rishabh Shrivastava Ronin, Manas Mathur, Garima Vaishnav, RS Sain, Parth Jhorar, Arbaj Pathan and Rakesh Kr Prajapat

Abstract:
The of variety CSV-17 of Sorghum bicolor seeds culture on different concentrations and combination of 2, 4-D, Kn, BAP, IAA, IBA, NAA was examined. The best callus volume were observed in 2.5 + 0.5 mg/L (2, 4-D and Kinetin). Copper nanoparticles (Cu NPs) were synthesized using the leaves of Moringa oleifera as reducing and capping agent and further characterized through UV-Visible spectroscopy and SEM. For subculturing, the fresh, viable and actively growing and proliferating calli pieces were weighed (1.0 g) and cultured on solid callus proliferation medium (MS) consisting of 2,4-D and Kn (2.0 + 0.5 mg/l)] with various doses of ABA and polyethylene glycol (control, 2, 6, 10 mg/L and control, 1.0, 1.5, 2 mg/L) respectively. Another media (MS) synergetically supplemented with Polyethylene glycol, ABA and copper nanoparticles doses (control, 1.0 + 2 + 5 mg/L; 1.5 + 6 + 10 mg/L; 2 + 10 + 20 mg/L) respectively and harvested for estimation of glycine-betaine and proline quantification after 6^th week. Positive effects were more considerable at its moderate dose ABA + PEG (6 + 1.5 mg/L); Cu-NPs + ABA + PEG (10 mg/L + 6 mg/L + 1.5 mg/L) and introducing it as the best treatment to ameliorate sorghum performance especially accumulation of proline and glycine betaine under drought stress. ABA and CuNps + ABA application at its higher dose (10; 10 + 20 mg/L) respectively demonstrated toxicity by negative impacts on accumulation of proline and glycine betaine. Plantlet regeneration occurred on MS containing BAP + Kn (2.0 + 0.3 mg/L); BAP + NAA (2.0 + 0.2 mg/L); BAP + Kn + NAA (2.1 + 1.0 + 0.3 mg/L) for shooting and IBA + NAA (3.5 + 0.4 mg/L) for rooting. In conclusion, the positive response of sorghum to ABA and ABA + CuNPs under drought stress conditions cause to consider that ABA and CuNps + ABA as potential novel plant growth promoting and stress protecting agent.