Vol. 12, Special Issue 11 (2023)

Author(s):
Shayista Akhter, Syed Shanaz, Ruksana Shah, Mudasir Andrabi, Nusrat Nabi, Mohsin Mir, Tavsief Ahmad, Riaz Shah and IU Sheikh

Abstract:
Prolactin is crucial for the growth of mammary glands, milk production, and milk protein gene expression. Principally produces lipids, lactose, and other significant milk constituents. It is a possible locus for quantitative traits and a genetic indicator of animal production traits. The current study screened local non-descript Gurezi cattle from Gurez Valley of Kashmir for Prolactin (PRL) gene by PCR-RFLP to explore potential genotypes of Gurez cattle. Gurezi cattle is an unexplored animal genetic resource that are exclusively found in the Gurez valley, situated in the Kishan Ganga basin in district of Bandipora. The population is well acclimated to the local agro-climatic conditions of area and reared primarily for milk and draught purpose by the Dardi tribes of Gurez Valley. On restriction digestion (Rsa I) of Prolactin gene (294 bp), three distinct banding patterns AA (a single band of 294 bp), Aa (three bands of 294/162/132 bp), and aa (two bands of 162/132 bp) were observed on gel (2.5%). The gene and genotype frequencies were estimated by using HWE formula. The population was found to be HWE and the genotype with higher frequency was found to be Aa, concluding that the Gurezi cattle shows polymorphism for PRL gene.