Vol. 12, Special Issue 12 (2023)

Author(s):
P Mounika, Dr. V Samatha, Dr. Ch. Sudha Rani Chowdary, Dr. G Srividya, Dr. V Rama Devi and Dr. V Prasad

Abstract:
The objective of this study was to carry out molecular diagnosis of Chicken Infectious Anemia in commercial chicken. A total of 143 commercial chicken from different farms located in different districts of AP were suspected for CIA based on history, clinical signs and gross lesions. On external examination, affected birds exhibited pale comb and wattles, atrophy of all lymphoid organs, pale discoloration of bone marrow along with hemorrhages on thigh and breast muscles. Blood samples collected randomly from the birds in the suspected flocks grossly appeared as pale and watery contents. On hematological examination there was a significant decline in the Hemoglobin and Packed Cell Volume indicating severe anemia in CIA infected birds. Molecular diagnosis of CIA was performed by polymerase chain reaction (PCR) by amplifying the VP1 and VP3 genes of CIAV.