Vol. 12, Special Issue 12 (2023)

Author(s):
Bolla Krishna, Bommi Reddy Sravani and Velpur Pavan Kumar

Abstract:
The studies were conducted to determine the efficacy of SANGUARD, a biosecurity product. In the first study, SANGUARD efficacy was tested by spraying SANGUARD @ 10 ml/Litre of water on the empty shed and the shed in the presence of 2000 birds at 39 days of age. Nutrient agar and Yeast and Mold Chloramphenicol agar plates were exposed for 1 minute in the empty shed and shed with birds and tested for the total bacterial and mold counts both before and after spraying SANGUARD. The total bacterial count and mold count were significantly reduced after spraying SANGUARD. In the second study, the water from different areas was collected and tested for pH and Most Probable Number (MPN) of organisms before and after adding SANGUARD @ 1 ml/10 L of water at 0, 2, 4, and 12 hrs time intervals. The result depicts that pH and MPN were reduced and maintained after adding SANGUARD @ 1 ml/ 10 L of water. In the third study, the efficacy of SANGUARD was tested against chlorine in Reverse Osmosis (RO) water. The colony-forming units (CFU) are observed at different time intervals by taking the sample from the RO water treated with chlorine tablet @ 45 mg and SANGUARD @ 2 ml separately. Results showed that the efficacy of SANGUARD was similar to that of chlorine.