Vol. 12, Special Issue 12 (2023)

Author(s):
Diganta Chetia, Subodh Gupta, Tincy Varghese, Showkat Ahmad Dar, Pallath Muhammed Nuzaiba, Gowher Iqbal, PP Srivastava and Parimal Sardar

Abstract:
A pharmacokinetics study of emamectin benzoate (EMB) was conducted for the first time in Indian major carp, Labeo rohita, and the physio-biochemical changes to the drug were studied. A single dose of EMB at 20 mg kg^-1 had administered through feed to L. rohita juveniles (100±10 g) and blood samples were collected at 0.5, 1, 2, 4, 8, 12, 24, 36, 48, 72, 96 and 120 hours after administration to determine the presence of EMB residue in plasma using C-18 reversed phase High-Performance Liquid Chromatography (HPLC). Maximum concentration (C_max) of 21.82±0.012 μg ml⁻¹ had detected at one hour (T_max). The depletion profile of emamectin benzoate indicated that the drug was eliminated in 96 h. The metabolic enzymes (ALT, AST, and LDH) and antioxidant enzymes (SOD, CAT, GST, GPx, and ALP) were significantly increased (p<0.05) in all EMB treatments groups compared to control group. All the above biomarkers were peaked on the 6^th day and gradually reduced up to the level of control by 15^th day, indicating that the physiological disturbance due to the drug was normalized within the above period. Also, the EROD assay, estimation of CYTP4501A induction was also proved to be a sensitive biomarker for indicating the pharmacodynamics response due to the drug, as the activity also showed the same trend as that of the enzymes.