Vol. 12, Special Issue 12 (2023)

Methods: This study involved the collection of blood samples from a cohort comprising 73 dogs, which were subsequently screened for the presence of Ehrlichia canis utilizing an innovative nested PCR assay. Positive samples identified through this screening process underwent genetic characterization, involving amplification and sequencing of a segment approximately 389 base pairs in length within the Ehrlichia Spp. 16S rRNA gene. The application of nested PCR was specifically employed to ascertain the species identification of Ehrlichia, strategically utilized to heighten the sensitivity and specificity of detecting E. canis within blood specimens.

Results: The utilization of the Nested PCR method proves highly sensitive in identifying Ehrlichia infections, even in subclinically affected dogs. Detection through this method prompts timely treatment recommendations for PCR-positive dogs, potentially serving as a life-saving intervention in numerous cases.

Conclusion: Drawing from the initial molecular evidence highlighting the presence of Ehrlichia canis in dogs, the suggested protocol stands poised to enhance the landscape of epidemiological investigations concerning canine ehrlichiosis. This innovative approach has the potential to significantly contribute to a more comprehensive understanding of the prevalence and spread of this disease among canine populations.