Vol. 12, Special Issue 12 (2023)

Author(s):
Maya Mehara, Deepika Goklaney, Manohar Lal Sain, Chandani Jawa, Rekha Loiya, Devendra Choudhary, Tapendra Kumar and Manju Kumari

Abstract:
A study was conducted on 90 biomedical waste samples obtained from a veterinary clinical complex to analyze the presence of bacterial species, their identification, and antibiogram. A total of 153 bacterial agents were isolated from these samples. Among them, 46 samples (51.11%) tested positive for E. coli, 32 samples (35.55%) tested positive for Staphylococcus aureus, 29 samples (32.22%) tested positive for Klebsiella spp., 20 samples (22.22%) tested positive for Pseudomonas aeruginosa, 14 samples (15.55%) tested positive for Bacillus spp., and 12 samples (13.33%) tested positive for Streptococcus spp. The antimicrobial resistance profile of these bacterial isolates was assessed against various antibiotics. The results revealed that the isolates exhibited sensitivity to gentamicin (90.84%), levofloxacin (88.88%), ciprofloxacin (86.27%), vancomycin (79.73%), co-trimoxazole (sulpha/trimethoprim) (78.43%), streptomycin (76.47%), and chloramphenicol (58.82%). However, the isolates showed resistance to ampicillin (86.27%), clindamycin (85.62%), oxacillin (84.96%), amoxyclav (84.96), erythromycin (80.39%), linezolid (79.73%), penicillin–G (56.20%), tetracycline (53.59%), and sulfatriad (53.59%). All the isolates exhibited resistance to at least five or six drugs, indicating a significant finding. Moreover, the Gram-negative bacteria demonstrated higher resistance to the antibiotics tested as compared to the Gram-positive bacteria.