Vol. 12, Special Issue 12 (2023)

Author(s):
Arun TS, VK Saxena and Vineetha PG

Abstract:
Effect of rearing systems on the gut micro biome of Indian male line broiler was investigated. Synthetic male line broiler developed at experimental broiler farm, CARI, Izatnagar was reared under intensive and extensive systems of rearing. Birds were humanely slaughtered at 5 weeks of age and whole intestinal contents were collected for NGS sequencing at Illumina (300 bp paired end) platform using primers targeting V3, V4, and V4-V6 hyper variable regions of 16srRNA. Microbial diversity was analysed using bioinformatics pipeline MG-RAST and Statistical differences for number of reads under various taxa revealed were analysed using Chi-square tests. The results revealed that gut micro biota differ significantly between rearing systems with total number of reads are quite high (334064) in chicks reared under extensive as compared to those reared under intensive system (198085).