Vol. 12, Special Issue 12 (2023)

Author(s):
Sandeep NC, Jess Vergis, Vinod VK, Asha K, Abishad P and Sanis Juliet

Abstract:
Aqueous extract of C. canephora robusta husk was used to synthesize ZnO nanoparticles as an innovative and sustainable strategy to combat the threat of multi-drug-resistant pathogens. The monsooned variety of Robusta coffee is a geographical indication of the district of Wayanad husk thereby a major agro-waste product produced during the processing and poses a significant environmental threat. Various bioactive molecules present in the coffee husk were thereby an excellent option for the green synthesis of Zinc oxide nanoparticles. An SPR (surface plasmon resonance) band at 374 nm confirmed the presence of zinc oxide. The antibacterial potential was evaluated through micro broth dilution assay and a minimum inhibitory concentration (MIC) of 1000 µg/mL and a minimum bactericidal concentration of 2000 µg/mL against the multi-drug resistant (MDR) test strains was observed. Therefore, the incorporation of nanotechnology to valorize an agro-waste product to combat the threat of MDR- pathogens was found to be effective.