Vol. 12, Special Issue 12 (2023)

Author(s):
Pandiri Keerthana, K Ramachandra Reddy, G Aruna Kumari, K Chandra Shekar Reddy and P Nagaraj

Abstract:
The present study was conduct to know the efficiency of CIDR + Ovsynch protocol for synchronisation of recipient cows for IVF (invitro fertilization) embryo transfer. IVEP (invitro embryo production) in combination with synchronisation and ET can play important role in faster multiplication of superior germplasm. 60 animals were selected randomly and assigned into 2 groups. Group1- Natural heat (n=20) cows exhibiting natural estrus were selected. Group 2- CIDR + Ovsynch (n=40) on day 0 CIDR was inserted + inj. GnRH- 10 µg, on day 7 CIDR removal + inj. Vetmate- 500 µg, on day 9 inj. GnRH- 10 µg. Embryo transfer was done in the recipient animals having CL by using non surgical method after 7-8 days of estrus by depositing the embryos in uterine horn ipsilateral to the ovary having CL. In the CIDR+ Ovsynch group 72.5% of animals exhibited estrus among these eligible recipients for transfer 86.2% and conception rate in embryo transferred were 13.23%. In natural estrus group, eligible recipients for transfer (83.3%) for transfer and its conception rate is 0%. There was a positive correlation (p<0.05) between the CL diameter and conception rate of recipient cows, the mean CL diameter of pregnant and non pregnant animals was 14.18±0.84 mm and 8.97±0.52 mm. In summary, higher synchronisation, eligible animals for transfer and conception rates were observed with timed ET in CIDR + Ovsynch protocol than in natural estrus animals.