Vol. 10, Special Issue 10 (2021)

Author(s):
K Narmada, P Amareswari, D Sakaram, P Kalyani and B Rajith Reddy

Abstract:
Leptin is one of the gene involved in immune functions, fertility and regulation of feed intake. In the present study 50 lactating Murrah buffaloes were screened to identify the polymorphism in exon-2 and exon-3 of leptin gene through PCR-RFLP by using 2 restriction enzymes MspI and Hind III. MspI had revealed two fragments in exon-2 (210bp, 79bp) and exon-3(255 bp, 150 bp). Whereas, Hind III did not reveal any cutting site in both the exons. PCR-RFLP patterns in the exon-2 and exon-3 with the two restriction enzymes employed revealed the monomorphic nature of the leptin gene so the production traits were not correlated with polymorphism as no allelic variants were found in the gene. The overall least squares mean for total lactation milk yield, standard lactation milk yield, lactation length, peak yield, attainment of peak yield were 1,943.77 ± 84.56 kg, 2008.27 ± 82.15kg, 291.13 ± 9.23 days, 11.88 ± 1.36 kg and 25.40 ± 1.28 days, respectively.