Vol. 10, Special Issue 11 (2021)

Author(s):
Kapil Dev, SS Dhaka, AS Yadav and Ankit Magotra

Abstract:
The present study was conducted to identify polymorphisms in Cacna2d1 gene and their association with production performance traits in Hardhenu cattle. The SNPs viz., A38795904G in intron 10 of Cacna2d1 gene were targeted and genotyped by PCR RFLP for the presence of polymorphism. Statistical analysis was performed to identify association of identified SNP with first lactation production performance traits in Hardhenu cattle. PCR amplified product of 386 bp harboring A38795904G mutation in particular intron 10 of Cacna2d1 gene resolved into three possible genotypes that were defined by three distinct banding patterns i.e. GG (229, 157 bp), AG (386, 229, 157 bp) and AA (386 bp) in present study. The genotypic frequency of GG genotype (GG= 62.5) with allelic frequencies as G = 0.79 was found to be predominant. While, corresponding allelic frequency of A (A=0.21) was reported to be rare in population at A38795904G locus. Chi-squared tests showed that A38795904G SNP meet with the Hardy–Weinberg equilibrium in our studied population. Least square analysis revealed significant association (p<0.01) between different genotypes of A38795904G SNP of Cacna2d1 gene with LMY-305 and FLL with the highest value of association of AG genotype with LMY-305 (2986.46±156.10 kg). While, it was highest for AA genotype with FLL (432.00±31.09 days). The association of this SNP with production performance traits can be utilized as an aid to selection after validation of results on large number of animals.