Vol. 10, Special Issue 11 (2021)

Author(s):
Ramkaran, Sandeep Gera, Ravi Kumar, Pradeep Kumar, Ankit Magotra and Sandeep Kumar

Abstract:
The heat shock protein (HSP) family is known for its role in cell protection. HSP 27, also known as heat shock beta 1, is involved in cellular organization (Creagh et al., 2000) and interacts with myofilaments (Blunt et al., 2007; Fischer et al., 2002). The present study was undertaken to to identify point variations in HSP 27 gene in 50 Hariana cattle. Genomic DNA was extracted from whole blood of lactating Hariana cattle. PCR amplification yielded products of 631bp for HSP 27 gene. Amplicons were subjected to direct sequencing and multiple sequence alignment using Clustal W revealed one nucleotide sequence variation in HSP 27 gene. In HSP 27 gene G225A was polymorphic with three genotypes AA, AB and BB in our resource population. Allele and genotype frequencies of HSP 27 gene were calculated using POPGENE software package. Genotype AA, BB and AB had frequencies 0.64, 0.28 and 0.08, respectively whereas, allele frequency for A and B allele were 0.78 and 0.22, respectively.