Vol. 10, Special Issue 11 (2021)

Author(s):
Ramkaran, Sandeep Gera, Ravi Kumar, Pradeep Kumar, Ankit Magotra and Sandeep Kumar

Abstract:
The 90 kDa heat shock protein family (HSP90) is one of the most abundant cytosolic proteins in eukaryotes. The present study was taken to explore the point variations in HSP90AB1 gene in 50 lactating Hariana cattle. A 459bp PCR fragment of HSP 90AB1 gene was amplified and amplicons were subjected to direct sequencing for characterization and mutation detection. Multiple sequence alignment using ClustalW of sequenced samples revelated but no SNP for HSP90AB1 gene in our studied population which indicates highly conserved DNA sequence in Hariana cattle. Since present study has formulated the results based on a relatively small sample, further studies are required to validate these results in large samples.