Vol. 10, Special Issue 11 (2021)

Author(s):
Anil Manga, K Venkataramana and K Muralimohan

Abstract:
The present study was conducted to determine the effects of the addition of antioxidants Ascorbic acid, BHT and Vit E in Tris-citric acid-fructose-egg yolk (TCFEY) to liquid Deccani Ram semen at refrigerated temperature (5 °c). Ejaculates were collected from eight Deccani rams by artificial vagina twice a week during non breeding season, after collection each ejaculate was diluted by Tris-citric acid-fructose-egg yolk (TCFEY), diluted semen samples was divided into four parts. The first part was added with Ascorbic acid (2mg/ml), second part added with Butylated hydroxy toluene (BHT) at the rate of 2mM/ml, Vitamin E was added at the rate of 2mg/ml to the third part and the fourth part was considered as control without any addition. The diluted semen samples were cooled gradually and preserved at 5 °C for three days. The sperms in cooled diluted semen samples were examined for motility, viability and abnormalities at 24, 48 and 72hrs of storage. the mean sperm motility percentage varied significantly (P<0.05) after 24hrs of storage at 5 °C which was found to be highest in Tris extender containing vitamin E followed by BHT ,Vitamin C and control group. After 48 and 72hrs of storage the individual motility was significantly highest in BHT followed by Vit E, Ascorbic acid and control. With regard to the total mean sperm viability was varied significantly highest in Tris extender containing BHT followed by Vit E, Ascorbic acid and control at 24,48 and 72hrs of storage .With regard to the mean sperm abnormalities varied significantly which was found to be lowest in Tris extender containing BHT followed by Vit E, Ascorbic acid and control at 24,48 and 72hrs of storage.