Vol. 10, Special Issue 11 (2021)

Author(s):
Robin, SK Jain and Pooja Kundu

Abstract:
Cypermethrin and deltamethrin are synthetic pyrethroid insecticide used in agriculture, home pest control and disease vector control. Pyrethroids cause oxidative stress and alteration in antioxidant enzymes in erythrocytes of pyrethroid intoxicated rats. Withania somnifera and resveratrol possesses anti-inflammatory, antitumor, anti-stress, antioxidant, immunomodulatory, hemopoetic, and rejuvenating properties. Myeloperoxidase (MPO) is an enzyme stored in azurophilic granules of polymorphonuclear cells and macrophages and released into extracellular fluid in the setting of inflammatory process. The assessment of MPO activity is well established for quantifying inflammation. In inflammatory conditions, the neutrophil levels of inflammed tissues, and consequently the MPO enzyme level is increased. The present study was undertaken to investigate effect of combined treatment of cypermethrin and deltamethrin on MPO (U/g tissue) levels in liver, kidney and brain and its amelioration by Withania somnifera and resveratrol in adult male Wistar rats in 14 and 28-days experimental study. Combined treatment of cypermethrin and deltamethrin significantly increased levels of MPO in tissues in both 14 and 28 days study as compared to naive which were reduced significantly by resveratrol and Withania somnifera co-treatment in cypermethrin and deltamethrin administered groups in both 14 as well as in 28 days. In conclusion, resveratrol co-treatment and Withania somnifera co-treatment reduced the MPO activity indicating its protective effect against cellular injury in adult male rats.