Vol. 10, Special Issue 11 (2021)

Author(s):
Jasleen Kaur, Gaurav Charaya, Sunil Punia, Praveen Kumar, Jinu Manoj and Rajesh Chhabra

Abstract:
Bacterial urinary tract infection in buffaloes is one of the important problem leading to decrease in production and reproduction status of a herd. Diagnosis and treatment is the major step in limiting the condition and its ill effects. Therefore, the present study was to determine the bacteria involved in urinary tract infection and to determine their antibiogram which can be used in field condition for empirical treatment. Buffaloes presented to teaching veterinary clinical complex during OPD hours were screened for UTI on the basis of clinical and microscopic examination of urine samples for pus cells. Out of 72 buffaloes, samples from 11 buffaloes collected by catheterization were subjected to cultural examination and determining antibiogram of isolates obtained. Out of eleven samples, mixed growth was evident in 72.72% and pure growth of single colony in 27.27% with isolation of 23 isolates. Staphylococcus sp. were the most isolated bacteria followed by Streptococcus sp., E. coli and single isolate of Klebsiella sp. Overall antimicrobial sensitivity revealed higher percentage of resistance with all the isolates being multi drug resistant. Among multidrug resistant isolates, 60.86% were extreme drug resistant and 30.43% were found to be pan drug resistant. Complete resistance was recorded against oxytetracyclin and streptomycin irrespective of the isolates. Gram negative bacteria were found to be completely resistant towards penicillin. Chloramphenicol was found to be most effective antimicrobials against Gram positive bacteria. Results of current study clearly depicts the importance of determination of antibiogram associated with bacterial urinary tract infection before initiating the therapy as empirical treatment can result in treatment failure in view of such a large proportion of multidrug resistance prevalent in field condition.