Vol. 10, Special Issue 12 (2021)

Author(s):
Dr. T Devi, M Asokkumar, M Vijaya Bharathi, A Ramesh and KG Tirumurugaan

Abstract:
A study was conducted in 30 numbers of Jersey cross cattle at Post Graduate Research Institute in Animal Sciences, Tamil Nadu Veterinary and Animal Sciences University for rapid detection of Mycobacteriosis. Lymph node smear and sputum swabs were collected from the study population and were subjected to Auramine -O-Fluorescent staining and Loop mediated isothermal Amplification (LAMP). Out of 30 samples, 16 (53.33%) samples were found as positive by both test methods with 22 samples (73.33%) sparing positive only by Auramine O Fluorescent staining. There were only 6 (20%) samples which showed negative to Auramine O Fluorescent staining but positive to LAMP method. It was concluded that these two diagnostic methods may be considered as parallel tests for rapid diagnosis of Mycobacteriosis where early diagnostic methods like Interferon Gamma assay are not feasible for isolation and quarantine of positive animals before confirmation by gold standard culture method.