Vol. 10, Special Issue 12 (2021)

Author(s):
Sudhanshu Raman, Ashutosh Dharmendra Deo, Md. Aklakur, Narottam Prasad Sahu, Yogananda T and Jyotismita Thakuria

Abstract:
The present study aimed to investigate the effect of combinatorial supplementation of bile acid, citric acid and xylanase enzymes on the growth performance of Labeo rohita fed with fermented sesbania leaf meal-based diet. Eighteen hapas were fixed in an earthen pond (2m×1m×1m dimension) and each hapa was stocked with 10 Labeo rohita juveniles (18.67±0.26 g.). Labeo rohita juveniles were fed with a basal diet containing fermented sesbania leaf-meal (30%) as a control. Four experimental diets T1, T2, T3 & T4 were supplemented with bile acid & citric acid, bile acid & xylanase, citric acid & xylanase and bile acid, citric acid & xylanase, respectively in the basal diet. The positive control group was fed with commercial carp feed. The result showed that weight gain percentage, specific growth rate (SGR) and FCR were significantly improved compare to the control group among all the treatment groups. The weight gain percentage was significantly higher in the Positive Control group (PC) (111.11%) and T4 (104.96%) with no significant difference among them. The least weight gain % recorded in control group (64.13%). In comparison to the positive control, T4 group did not illustrate any significant difference (P>0.05) in any growth parameters like weight gain percentage, specific growth rate (SGR) and FCR. Treatments supplemented with a combination of bile acid, xylanase and citric acid showed enhanced lipase & amylase activity along with increased bioavailability of minerals. The present study has demonstrated that dietary bile acid, citric acid and xylanase enzyme supplementation in fermented sesbania leaf meal-based diet led to enhanced utilization of nutrients from plant ingredient-based diet and improved the growth performance of Labeo rohita.