Vol. 10, Special Issue 12 (2021)

Author(s):
Chandrashekhara N, Shrikrishna Isloor, Rathnamma D, Thyageeswaran, Veeregowda BM, Shridhar NB, Roopa Devi YS and Pavan Kumar KN

Abstract:
The study was carried out to evaluate humoral immune response with reference to serum IgG level by indirect ELISA, in mastitis causing E. coli Biofilm (BF) vaccinated and Free cell (FC) vaccinated groups. In all, eighteen cattle in early lactation which were free from mastitis were subjected to trials. Bentonite clay (an adjuvant by itself) based E. coli BF and FC vaccines were administered at 0, 30 and 60 days. Freund’s incomplete adjuvant was incorporated in the first shot. Both BF and FC vaccinated groups showed significant difference with control group T cell proliferative immunological response by analyzing humoral immune responses indicated superiority of bovine mastitis causing E.coli BF vaccine over FC vaccine.