Vol. 10, Special Issue 12 (2021)

Author(s):
C Sworna Kumari, TV Meenambigai and A Mangalagowri

Abstract:
The immunogenic protein VP2 of highly virulent infectious Bursal disease virus (hvIBDV) strain were cloned and expressed in mammalian expression vector pVAX.1. The pVAX.1 vector, has high-copy number replication in E. coli with high-level transient expression of the protein of interest in most mammalian cells due to the presence of human cytomegalovirus (CMV) promoter. VP2 protein was transformed in E. coli BL21 DE3 host system and characterized by SDS PAGE and western blotting. The present paper focuses on the strategies of cloning and expression of the immunodominant protein of IBDV which in future can be used as a DNA vaccine to control IBDV infection in chickens.